| 1. | However , the homologies of g and n gene between wild strains and attenuated strain ctn are much high
三株野毒与减毒株ctn之间的n和g基因同源性均高于与3ag 、 era株的同源性。 |
| 2. | In this study three wild strains of rabies virus were isolated from nanning , longan and bama of guangxi and were designated as 119 , gxla and gxbm respectively
本试验从广西南宁、隆安和巴马分离到三株狂犬病毒野毒株，分别编号为119 、 gxla和gxbm 。 |
| 3. | A few mutants were found on the plate containing nitrobenzene selective medium . their colony appearances have some difference from the wild strain . some evidences show that their growth characteristics on the selective medium are related to the plasmids harbored in their cells
对菌株的自发突变株的研究表明，这些菌株在硝基苯选择培养基上的生长特性是与它们细胞中所含质粒的特性相关的。 |
| 4. | The plasmid extraction from wild strains needs an appropriate method . first , lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1 . but the expected result had not been got , and a lot of cracked plasmid dna fragments were got only
对于野生菌株的质粒提取，需要选择合适的提取方法，我们首先采用了细菌细胞质粒提取常用的碱裂解法，但是未取得理想的效果，只得到了许多破碎的质粒dna片段。 |
| 5. | Because of difficulty in differentiating eiav wild strain from the vaccine strain （ dla ） in vivo ， we have to build a fast identification method between wild strain and vaccine strain of eiav s2 is an accessory protein of equine infectious anemia virus ( eiav ) , the s2 function is not fully defined
由于我国马传贫弱毒疫苗存在野毒和疫苗毒鉴别困难的问题，目前尚未得到欧美等发达国家的认证，如要进入国际市场，急需建立一种eiav强、弱毒快速鉴别诊断方法。 |
| 6. | However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration
植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。 |
| 7. | However , the content of coq10 in wild strains of psb is limited after all . through construction of recombinant strain producing coq10 and optimization of the culture medium and fermentation conditions of a selected strain , the content of coq10 in psb will be increased markedly . it can not only remarkably strengthen the effect of psb feed additive , but also lay foundation for the exploitation of coq10
但野生型psb菌株coq _ ( 10 )含量终究有限，如能选择合适菌株进行培养优化，并通过构建基因工程菌，使菌体中coq _ ( 10 )的含量成倍提高，则不仅可显著增强psb饲料添加剂的应用效果，更重要的是，为coq _ ( 10 )相关产品的研发奠定良好的基础。 |
| 8. | However , its wide applications in manufacture are restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means in which using an efficient expression system to express heterogenous phytase is a main consideration
但微生物野生菌株产酶水平较低，制约了微生物植酸酶在生产上的广泛应用，因此提高植酸酶野生菌株的产酶水平是实现其广泛应用的关键所在。目前，提高野生菌株产酶水平的主要思路是通过基因工程手段克隆其植酸酶基因，然后在生物反应器中高效表达该植酸酶基因。 |
